Fgf8^tm1.2Mrt
Targeted Allele Detail

Summary

• Symbol: Fgf8^tm1.2Mrt
• Name: fibroblast growth factor 8; targeted mutation 1.2, Gail R Martin
• MGI ID: MGI:2150346
• Synonyms: Fgf8^delta2,3n
• Gene: Fgf8 Location: Chr19:45725237-45731354 bp, - strand Genetic Position: Chr19, 38.75 cM, cytoband C3-D
• Alliance: Fgf8^tm1.2Mrt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:45909
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele is a derivative of Fgf8^tm1.1Mrt in which mice carrying this allele were mated to beta-actin cre mice, which resulted in heterozygous offspring carrying a recombined Fgf8 allele in which exons 2 and 3 were deleted. (J:45909)

Expression

In Structures Affected by this Mutation:

25 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fgf8 Mutation: 21 strains or lines available

Notes

One of three alleles produced using a targeting construct capable of inducing different allelic variants when mated to mice that express cre, flp, or both. Referred to in the literature as Fgf8^delta2,3n, this allele lacks exons 2 and 3 and is a presumed null. Heterozygotes are normal. Homozygotes are smaller than littermates and lack all embryonic mesoderm- derived structures including heart and somites. This mutation causes lethality by embryonic day 10.5 (J:45909).

References

• Original: J:45909 Meyers EN, et al., An Fgf8 mutant allelic series generated by Cre- and Flp-mediated recombination. Nat Genet. 1998 Feb;18(2):136-41
• All: 19 reference(s)