Hoxa5^tm1Rob
Targeted Allele Detail

Summary

• Symbol: Hoxa5^tm1Rob
• Name: homeobox A5; targeted mutation 1, Elizabeth J Robertson
• MGI ID: MGI:2150397
• Synonyms: Hoxa5-
• Gene: Hoxa5 Location: Chr6:52178768-52181437 bp, - strand Genetic Position: Chr6, 25.4 cM
• Alliance: Hoxa5^tm1Rob page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:15576
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Insertion of a neomycin resistance cassette into exon 2 disrupted sequences that encode the DNA binding homeodomain. Northern analysis using an exon 2 probe detected novel transcripts and no wild-type transcripts in lung and kidney of homozygous mutant mice. (J:71563)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)

	Allelic Composition	Genetic Background	Cell Line(s)
Loading...
hm1	Hoxa5^tm1Rob/Hoxa5^tm1Rob	129S(Cg)-Hoxa5^tm1Rob
hm2	Hoxa5^tm1Rob/Hoxa5^tm1Rob	B6.129S(Cg)-Hoxa5^tm1Rob
hm3	Hoxa5^tm1Rob/Hoxa5^tm1Rob	involves: 129S/SvEv * C57BL/6 * MF1
hm4	Hoxa5^tm1Rob/Hoxa5^tm1Rob	involves: 129S/SvEv * MF1
cx5	Hoxa5^tm1Rob/Hoxa5^tm1Rob Pdgfra^tm11(EGFP)Sor/Pdgfra^+	involves: 129S/SvEv * 129S4/SvJaeSor * C57BL/6 * MF1
cx6	Hoxa5^tm1Rob/Hoxa5^+ Hoxa6^tm1Mrc/Hoxa6^+ Hoxb5^tm1Mrc/Hoxb5^+ Hoxb6^tm1Mrc/Hoxb6^+ Hoxc5^tm1Mrc/Hoxc5^+ Hoxc6^tm1Dds/Hoxc6^+	involves: 129S/SvEv * 129S7/SvEvBrd
cx7	Hoxa5^tm1Rob/Hoxa5^tm1Rob Hoxb5^tm1Mrc/Hoxb5^tm1Mrc Hoxc5^tm1Mrc/Hoxc5^tm1Mrc	involves: 129S/SvEv * 129S7/SvEvBrd

Phenotypes:

Affected Systems	hm1		hm2	hm3	hm4	cx5	cx6	cx7
show or hide all annotated terms Sex:
cellular						√
abnormal cell migration						√
craniofacial	√	√
delayed ear emergence	√	√
digestive/alimentary system	√	√	√
abnormal digestive secretion	√	√
meteorism	√	√	√
endocrine/exocrine glands	√	√
abnormal thyroid gland development	√	√
ovary cyst	√
growth/size/body	√	√	√
delayed ear emergence	√	√
meteorism	√	√	√
ovary cyst	√
postnatal growth retardation	√	√	√
hearing/vestibular/ear	√	√
delayed ear emergence	√	√
hematopoietic system				√
abnormal alveolar macrophage morphology				√
homeostasis/metabolism	√	√
increased circulating estradiol level	√
increased circulating thyroid-stimulating hormone level	√	√
cyanosis	√	√
immune system				√
abnormal alveolar macrophage morphology				√
lung inflammation				√
mortality/aging	√	√	√		√
neonatal lethality, incomplete penetrance	√	√	√
postnatal lethality, incomplete penetrance	√	√	√		√
reproductive system	√
ovary cyst	√
early sexual maturation	√
abnormal estrous cycle	√
prolonged diestrus	√
short estrus	√
prolonged metestrus	√
prolonged estrous cycle	√
respiratory system	√	√	√	√		√
lung inflammation				√
abnormal larynx morphology	√	√
abnormal cricoid cartilage morphology	√	√	√
abnormal branching involved in lung morphogenesis	√	√
impaired branching involved in bronchus morphogenesis			√
impaired branching involved in respiratory bronchiole morphogenesis	√	√
impaired lung alveolus development				√
abnormal lung saccule morphology	√	√
abnormal bronchus epithelium morphology	√	√
abnormal lung weight				√
abnormal pulmonary acinus morphology	√	√
abnormal pulmonary alveolus morphology				√
increased type II pneumocyte number				√
atelectasis	√	√	√	√
overexpanded pulmonary alveolus				√
abnormal pulmonary interalveolar septum morphology				√
thick pulmonary interalveolar septum			√
emphysema				√
abnormal pulmonary alveolar parenchyma morphology				√		√
abnormal alveolar macrophage morphology				√
abnormal pulmonary elastic fiber morphology				√
abnormal bronchiole morphology	√	√
abnormal bronchiole epithelium morphology	√	√
abnormal respiratory mucosa goblet cell morphology				√
increased respiratory mucosa goblet cell number				√
abnormal trachea morphology	√	√	√
abnormal tracheal cartilage morphology	√	√
cricoid and tracheal cartilage fusion	√	√
decreased tracheal cartilage ring number	√	√	√
trachea occlusion	√	√	√
trachea stenosis	√	√
respiratory distress	√	√
abnormal surfactant secretion				√
decreased surfactant secretion	√	√	√
skeleton	√	√	√		√		√	√
abnormal skeleton morphology							√
abnormal tracheal cartilage morphology	√	√
decreased tracheal cartilage ring number	√	√	√
abnormal axial skeleton morphology								√
increased sternebra number					√
sternebra fusion					√
absent sternal manubrium								√
short sternum								√
abnormal rib joint morphology								√
asymmetric sternocostal joints					√
abnormal rib morphology					√
abnormal rib development								√
decreased rib number								√
increased rib number					√
rib fusion					√			√
small thoracic cage								√
abnormal intervertebral disk morphology								√
abnormal cricoid cartilage morphology	√	√	√
cricoid and tracheal cartilage fusion	√	√
abnormal vertebrae morphology					√
cervical vertebral fusion								√
vertebral transformation								√
thoracic vertebral transformation								√
cervical vertebral transformation					√			√
lumbar vertebral transformation					√
vision/eye	√	√	√
delayed eyelid opening	√	√	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:	217 assay results
In Structures Affected by this Mutation:	23 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Hoxa5^tm1Rob

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hoxa5 Mutation: 28 strains or lines available

References

• Original: J:15576 Jeannotte L, et al., Specification of axial identity in the mouse: role of the Hoxa-5 (Hox1.3) gene. Genes Dev. 1993 Nov;7(11):2085-96
• All: 27 reference(s)