Sema3c^tm1.1Jra
Targeted Allele Detail

Summary

• Symbol: Sema3c^tm1.1Jra
• Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C; targeted mutation 1.1, Jonathan A Raper
• MGI ID: MGI:2150759
• Synonyms: Sema3C^-, Sema3C(neo^out)
• Gene: Sema3c Location: Chr5:17779814-17935266 bp, + strand Genetic Position: Chr5, 7.99 cM
• Alliance: Sema3c^tm1.1Jra page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71240
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: This allele is a derivative of Sema3c^tm1Jra in which the neomycin resistance gene was removed by Cre mediated recombination by crossing mice that carry the Sema3c allele to mice that express Cre recombinase in the germ cell. mRNA in homozygous mutant E12.5 embryos was barely detectable by in situ hybridization. RT-PCR analysis of these embryos detected transcript from regions 5' and 3' of the targeted sequences, but did not detect transcript from a region internal to the targeted sequences. (J:71240)

Expression

In Mice Carrying this Mutation:	18 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sema3c Mutation: 46 strains or lines available

References

• Original: J:71240 Feiner L, et al., Targeted disruption of semaphorin 3C leads to persistent truncus arteriosus and aortic arch interruption. Development. 2001 Aug;128(16):3061-70
• All: 10 reference(s)