Scx^tm1Eno
Targeted Allele Detail

Summary

• Symbol: Scx^tm1Eno
• Name: scleraxis scleraxis bHLH transcription factor; targeted mutation 1, Eric N Olson
• MGI ID: MGI:2150910
• Synonyms: scleraxis -
• Gene: Scx Location: Chr15:76341652-76343658 bp, + strand Genetic Position: Chr15, 35.92 cM
• Alliance: Scx^tm1Eno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:55991
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S7/SvEvBrd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A LacZ-PGK-neomycin resistance cassette inserted in-frame with the initiation codon replaced all except the 13 most distal residues of the first exon, which encodes the bHLH region. RT-PCR and whole mount in situ studies did not detect transcripts in homozygous mutant embryos. The lacZ reporter is non-functional. (J:55991)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Scx Mutation: 15 strains or lines available

Notes

ES cell line = AB1 (129S7/SvEvBrd-Hprt⁺) or AB2.2 (129S7/SvEvBrd-Hprt^b-m2)

References

• Original: J:55991 Brown D, et al., Dual role of the basic helix-loop-helix transcription factor scleraxis in mesoderm formation and chondrogenesis during mouse embryogenesis. Development. 1999 Oct;126(19):4317-29
• All: 1 reference(s)