Hcrt^tm1Ywa
Targeted Allele Detail

Summary

• Symbol: Hcrt^tm1Ywa
• Name: hypocretin; targeted mutation 1, Masashi Yanagisawa
• MGI ID: MGI:2151182
• Synonyms: OKO, orexin knockout, orexin KO, orexin-tau-LacZ, ORX-KO, Ox^-
• Gene: Hcrt Location: Chr11:100652519-100653757 bp, - strand Genetic Position: Chr11, 63.6 cM
• Alliance: Hcrt^tm1Ywa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:57124
• Parent Cell Line: SM1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin-lacZ cassette replaced exon 1 of the gene. In situ hybridization, immunohistochemistry, and radioimmunoassay of homozygous mutant mice brain failed to detect either orexin-A or orexin-B peptides. (J:57124, J:136109)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Hcrt Mutation: 19 strains or lines available

References

• Original: J:57124 Chemelli RM, et al., Narcolepsy in orexin knockout mice: molecular genetics of sleep regulation. Cell. 1999 Aug 20;98(4):437-51
• All: 76 reference(s)