Gnaq^tm1Soff
Targeted Allele Detail

Summary

• Symbol: Gnaq^tm1Soff
• Name: guanine nucleotide binding protein, alpha q polypeptide; targeted mutation 1, Stefan Offermanns
• MGI ID: MGI:2151661
• Synonyms: Galphaq-, Gnaq^-
• Gene: Gnaq Location: Chr19:16110195-16364827 bp, + strand Genetic Position: Chr19, 11.01 cM
• Alliance: Gnaq^tm1Soff page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:44598
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced 2.3 kb of sequence containing an exon that encodes amino acids 246-297 of the protein. Western blot analysis of whole brain and of cerebellar membrane fractions did not detect any protein produced from this allele in homozygous mice. (J:44598)

Expression

In Mice Carrying this Mutation:

3 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gnaq Mutation: 24 strains or lines available

References

• Original: J:44598 Offermanns S, et al., Impaired motor coordination and persistent multiple climbing fiber innervation of cerebellar Purkinje cells in mice lacking Galphaq. Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):14089-94
• All: 48 reference(s)