Hoxb1^tm2Brd
Targeted Allele Detail

Summary

• Symbol: Hoxb1^tm2Brd
• Name: homeobox B1; targeted mutation 2, Allan Bradley
• MGI ID: MGI:2151770
• Synonyms: Hoxb-1^-
• Gene: Hoxb1 Location: Chr11:96256578-96259082 bp, + strand Genetic Position: Chr11, 59.86 cM
• Alliance: Hoxb1^tm2Brd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:37450
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: Insertion of an in-frame lacZ gene and neomycin cassette into the sequences encoding the first exon. A frameshift mutation at an Sst1 site in the sequences encoding the homeobox domain was also made by cutting with the enzyme, blunt ending and ligating back together. No wild-type protein was detected in E9.5 embryos as assayed by whole mount immunohistochemisty. (J:37450)

Expression

In Mice Carrying this Mutation:	23 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hoxb1 Mutation: 24 strains or lines available

References

• Original: J:37450 Studer M, et al., Altered segmental identity and abnormal migration of motor neurons in mice lacking Hoxb-1. Nature. 1996 Dec 19-26;384(6610):630-4
• All: 2 reference(s)