Gna15^tm1Tmw
Targeted Allele Detail

Summary

• Symbol: Gna15^tm1Tmw
• Name: guanine nucleotide binding protein, alpha 15; targeted mutation 1, Thomas M Wilkie
• MGI ID: MGI:2152420
• Gene: Gna15 Location: Chr10:81338140-81360059 bp, - strand Genetic Position: Chr10, 39.72 cM
• Alliance: Gna15^tm1Tmw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:59992
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced 8kb of sequence that contains exons 3 through 6. The sequences encode three of the five amino acid motifs which are essential for guanine nucleotide binding to the alpha subunit. Western blot analysis did not detect any protein produced from this allele in extracts derived from spleen, thymus, lymph nodes or lungs of homozygous mutant mice. (J:59992)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gna15 Mutation: 31 strains or lines available

References

• Original: J:59992 Davignon I, et al., Normal hematopoiesis and inflammatory responses despite discrete signaling defects in Galpha15 knockout mice. Mol Cell Biol. 2000 Feb;20(3):797-804
• All: 5 reference(s)