Map1b^tm1Noh
Targeted Allele Detail

Summary

• Symbol: Map1b^tm1Noh
• Name: microtubule-associated protein 1B; targeted mutation 1, Nobutaka Hirokawa
• MGI ID: MGI:2152588
• Synonyms: MAPP1B-, R21
• Gene: Map1b Location: Chr13:99557954-99653048 bp, - strand Genetic Position: Chr13, 52.9 cM
• Alliance: Map1b^tm1Noh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:41889
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Insertion of a PGK-neomycin resistance cassette into the first coding exon disrupted the light-chain binding domain of the gene. The predicted truncated product of this allele is 11 amino acids long. Immunoblot analyses of brain using antisera to the microtubule-binding domain, to the carboxy-terminal imperfect repeats region, and to phosphorylated protein, detected little or no wild-type protein in homozygous mutant mice at postnatal day 4 and at 8 weeks of age. (J:41889)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Map1b Mutation: 85 strains or lines available

References

• Original: J:41889 Takei Y, et al., Delayed development of nervous system in mice homozygous for disrupted microtubule-associated protein 1B (MAP1B) gene. J Cell Biol. 1997 Jun 30;137(7):1615-26
• All: 3 reference(s)