Gnas^tm1Gwa
Targeted Allele Detail

Summary

• Symbol: Gnas^tm1Gwa
• Name: GNAS complex locus; targeted mutation 1, Gary Wand
• MGI ID: MGI:2152744
• Synonyms: Gnas^E1-
• Gene: Gnas Location: Chr2:174126113-174188537 bp, + strand Genetic Position: Chr2, 97.89 cM, cytoband E1-H3
• Alliance: Gnas^tm1Gwa page

Subcutaneous ossifications in Gnas^tm1Gwa/Gnas⁺ mice

Show the 6 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70175
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced 0.5kb of sequence containing the ATG codon and extending through an NruI site in intron 1. Quantitative immunoblot studies showed protein levels in multiple brain regions of mice heterozygous for this allele were significantly reduced relative to wild-type. (J:70175)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gnas Mutation: 54 strains or lines available

References

• Original: J:70175 Wand G, et al., The cAMP-protein kinase A signal transduction pathway modulates ethanol consumption and sedative effects of ethanol. J Neurosci. 2001 Jul 15;21(14):5297-303
• All: 7 reference(s)