Map1b^tm1Rak
Targeted Allele Detail

Summary

• Symbol: Map1b^tm1Rak
• Name: microtubule-associated protein 1B; targeted mutation 1, Raju Kucherlapati
• MGI ID: MGI:2152745
• Synonyms: Map1b571
• Gene: Map1b Location: Chr13:99557954-99653048 bp, - strand Genetic Position: Chr13, 52.9 cM
• Alliance: Map1b^tm1Rak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:31721
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Insertion of a PGK-neomycin resistance cassette in reverse transcriptional orientation created a premature termination codon corresponding to codon 571 ahead of the microtubule binding domain. Immunohistochemical studies of heterozygous mutant mice revealed significant changes in protein expression in several parts of the brain, suggesting that this is a null allele. (J:31721)

Expression

In Structures Affected by this Mutation:

10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Map1b Mutation: 85 strains or lines available

References

• Original: J:31721 Edelmann W, et al., Neuronal abnormalities in microtubule-associated protein 1B mutant mice. Proc Natl Acad Sci U S A. 1996 Feb 6;93(3):1270-5
• All: 2 reference(s)