Ntf3^tm1(Bdnf)Tes
Targeted Allele Detail

Summary

• Symbol: Ntf3^tm1(Bdnf)Tes
• Name: neurotrophin 3; targeted mutation 1, Lino Tessarollo
• MGI ID: MGI:2152961
• Synonyms: B/N
• Gene: Ntf3 Location: Chr6:126078375-126143703 bp, - strand Genetic Position: Chr6, 60.45 cM
• Alliance: Ntf3^tm1(Bdnf)Tes page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:72304
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Mutation details: Sequences encoding the BDNF protein replaced the entire coding region of the Ntf3 gene. A loxP flanked neomycin cassette was inserted adjacent to the coding region of the gene, but was removed via cre-mediated recombination in the final allele. Northern blot analysis and in situ hybridization analysis confirmed expression of a Bdnf transcript from this allele that mimicked the expression pattern of the endogenous Ntf3. (J:72304)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ntf3 Mutation: 24 strains or lines available

References

• Original: J:72304 Coppola V, et al., Dissection of NT3 functions in vivo by gene replacement strategy. Development. 2001 Nov;128(21):4315-27
• All: 3 reference(s)