Gnat3^tm1Rfm
Targeted Allele Detail

Summary

• Symbol: Gnat3^tm1Rfm
• Name: G protein subunit alpha transducin 3; targeted mutation 1, Robert F Margolskee
• MGI ID: MGI:2152966
• Synonyms: alpha-gus^-, alpha-gustducin null, Gust KO
• Gene: Gnat3 Location: Chr5:18167568-18224666 bp, + strand Genetic Position: Chr5, 8.14 cM
• Alliance: Gnat3^tm1Rfm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:36885
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Insertion of a PGK-neomycin resistance cassette into the first protein coding exon replaced the first protein coding region including the ATG initiation region and the splice donor sequences. RNA probes generated from exon 8 sequences did not detect message in taste receptor cells of homozygous mutant mice. (J:36885)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gnat3 Mutation: 22 strains or lines available

References

• Original: J:36885 Wong GT, et al., Transduction of bitter and sweet taste by gustducin [see comments]. Nature. 1996 Jun 27;381(6585):796-800
• All: 22 reference(s)