Thbs2^tm1Bst
Targeted Allele Detail

Summary

• Symbol: Thbs2^tm1Bst
• Name: thrombospondin 2; targeted mutation 1, Paul Bornstein
• MGI ID: MGI:2153006
• Synonyms: Thbs2^-, Tsp-2^-, TSP2-
• Gene: Thbs2 Location: Chr17:14885762-14914497 bp, - strand Genetic Position: Chr17, 8.95 cM, cytoband A3-B
• Alliance: Thbs2^tm1Bst page

Thbs2^tm1Bst/Thbs2^tm1Bst mice show increased flexibility of tail tendons and ligaments such that a knot can be tied in the tail

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:45509
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced a 2.6kb genomic fragment containing exons 2 and 3. Northern blot analysis demonstrated that no transcript was produced from this allele and Western blot analysis revealed that the protein was not detectable in homozygous E17 embryos. (J:45509)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Thbs2 Mutation: 68 strains or lines available

Notes

ES cell line = J1 (129S4/SvJae) or R1 ((129X1/SvJ x 129S1/Sv)F1-Kitl⁺).

References

• Original: J:45509 Kyriakides TR, et al., Mice that lack thrombospondin 2 display connective tissue abnormalities that are associated with disordered collagen fibrillogenesis, an increased vascular density, and a bleeding diathesis. J Cell Biol. 1998 Jan 26;140(2):419-30
• All: 53 reference(s)