Sparcl1^tm1Pmc
Targeted Allele Detail

Summary

• Symbol: Sparcl1^tm1Pmc
• Name: SPARC-like 1; targeted mutation 1, Peter J McKinnon
• MGI ID: MGI:2153047
• Synonyms: hevin null
• Gene: Sparcl1 Location: Chr5:104226977-104261599 bp, - strand Genetic Position: Chr5, 50.55 cM, cytoband E4
• Alliance: Sparcl1^tm1Pmc page

Abnormal Purkinje cell morphology in the Sparcl1^tm1Pmc/Sparcl1^tm1Pmc and Adamts4^tm1Dqp/Adamts4^tm1Dqp cerebellum

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:59207
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin cassette replaced exon 2, which encodes the secretion signal sequence. RNAse protection assays demonstrated an absence of transcript in RNA derived from cerebellum of adult homozygous mice. Western blot and immunohistochemical analysis did not detect the protein in brain of homozygous mice. (J:59207)

Expression

In Mice Carrying this Mutation:

3 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sparcl1 Mutation: 42 strains or lines available

References

• Original: J:59207 McKinnon PJ, et al., Extracellular matrix-associated protein Sc1 is not essential for mouse development. Mol Cell Biol. 2000 Jan;20(2):656-60
• All: 14 reference(s)