Matn1^tm1Ref
Targeted Allele Detail

Summary

• Symbol: Matn1^tm1Ref
• Name: matrilin 1, cartilage matrix protein; targeted mutation 1, Reinhard Fassler
• MGI ID: MGI:2153051
• Synonyms: Crtm -, Matn1^-
• Gene: Matn1 Location: Chr4:130671696-130682786 bp, + strand Genetic Position: Chr4, 64.09 cM
• Alliance: Matn1^tm1Ref page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67300
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A genomic fragment containing part of exon 3 and exons 4-6 was replaced by a neomycin resistance cassette. Northern blot analysis of total RNA derived from limb and cartilages demonstrated an absence of transcript in homozygous mice. Immunohistochemistry and Western blot analyses confirmed the lack of the protein in the cartilage of homozygous mice. (J:67300)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Matn1 Mutation: 20 strains or lines available

References

• Original: J:67300 Aszodi A, et al., Normal skeletal development of mice lacking matrilin 1: redundant function of matrilins in cartilage?. Mol Cell Biol. 1999 Nov;19(11):7841-5
• All: 5 reference(s)