Neurl1a^tm1Led
Targeted Allele Detail

Summary

• Symbol: Neurl1a^tm1Led
• Name: neuralized E3 ubiquitin protein ligase 1A; targeted mutation 1, Philip Leder
• MGI ID: MGI:2153143
• Gene: Neurl1a Location: Chr19:47167259-47247880 bp, + strand Genetic Position: Chr19, 39.39 cM
• Alliance: Neurl1a^tm1Led page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:72105
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: Insertion of an IRES-GFP-PGK-neomycin resistance cassette into the exon encoding the NHR-2 region of the protein disrupted the gene and introduced transcriptional termination sequences downstream of the GFP coding sequences. Northern analyses of brain and skeletal muscle of homozygous mutant mice detected only aberrant transcripts using a 5' probe, and did not detect transcript using a 3' probe. (J:72105)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Neurl1a Mutation: 20 strains or lines available

References

• Original: J:72105 Vollrath B, et al., Isolation of a Murine Homologue of the Drosophila neuralized Gene, a Gene Required for Axonemal Integrity in Spermatozoa and Terminal Maturation of the Mammary Gland. Mol Cell Biol. 2001 Nov;21(21):7481-94
• All: 1 reference(s)