Otx2^tm2(Otx1)Sia
Targeted Allele Detail

Summary

• Symbol: Otx2^tm2(Otx1)Sia
• Name: orthodenticle homeobox 2; targeted mutation 2, Shinichi Aizawa
• MGI ID: MGI:2153203
• Synonyms: Otx2^Otx1
• Gene: Otx2 Location: Chr14:48894238-48905101 bp, - strand Genetic Position: Chr14, 25.36 cM
• Alliance: Otx2^tm2(Otx1)Sia page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:51989
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: A murine Otx1 cDNA was inserted at the translation start site of the Otx2 gene. An adjacent loxP-flanked neomycin resistance cassette was removed in zygotes from crosses of the chimeric males and C57BL/6 females by injection of a cre cDNA under the control of the beta-actin promoter to produce F1 mice in which the neo cassette was deleted. This allele expresses Otx1 under the control of the Otx2 promoter elements. (J:51989)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Otx2 Mutation: 50 strains or lines available

References

• Original: J:51989 Suda Y, et al., Functional equivalency between Otx2 and Otx1 in development of the rostral head. Development. 1999 Feb;126(4):743-57
• All: 1 reference(s)