Fgfr1^tm1Cxd
Targeted Allele Detail

Summary

• Symbol: Fgfr1^tm1Cxd
• Name: fibroblast growth factor receptor 1; targeted mutation 1, Chu-Xia Deng
• MGI ID: MGI:2153353
• Synonyms: Fgfr1^ex3
• Gene: Fgfr1 Location: Chr8:26008808-26067819 bp, + strand Genetic Position: Chr8, 14.12 cM
• Alliance: Fgfr1^tm1Cxd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:54222
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced exon 3, which encodes the first Ig domain contained only in alpha isoforms of the protein. Western blot analysis demonstrated that the alpha isoforms of the protein were not expressed in E9.5 mutant embryos. RT-PCR analysis showed that an increased level of expression of "b" isoform transcripts may partially compensate for loss of alpha isoform expression, and the expression of the "c" isoform is apparently unaffected. (J:54222)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fgfr1 Mutation: 223 strains or lines available

References

• Original: J:54222 Xu X, et al., Murine fibroblast growth factor receptor 1alpha isoforms mediate node regression and are essential for posterior mesoderm development. Dev Biol. 1999 Apr 15;208(2):293-306
• All: 3 reference(s)