Creb1^tm1Gsc
Targeted Allele Detail

Summary

• Symbol: Creb1^tm1Gsc
• Name: cAMP responsive element binding protein 1; targeted mutation 1, Gunther Schutz
• MGI ID: MGI:2153538
• Synonyms: CREB -, CREB^alphadelta-, CREB^alphadelta, CREBalphadelta
• Gene: Creb1 Location: Chr1:64571963-64643707 bp, + strand Genetic Position: Chr1, 32.74 cM, cytoband C1/C5
• Alliance: Creb1^tm1Gsc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:18749
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A promoterless neomycin resistance gene was inserted in frame into exon 2. RNase protection assays on RNA derived from liver of homozyogous mice demonstrated that no detectable alpha or delta isoform transcript was produced from this allele; however, a beta isoform transcript is upregulated (J:31886). Western blot analysis on liver extracts from homozygous mice confirmed that no alpha or delta isoform of the encoded protein was produced. (J:18749, J:31886)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Creb1 Mutation: 61 strains or lines available

References

• Original: J:18749 Hummler E, et al., Targeted mutation of the CREB gene: compensation within the CREB/ATF family of transcription factors. Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5647-51
• All: 37 reference(s)