Fgfr2^tm1Cxd
Targeted Allele Detail

Summary

• Symbol: Fgfr2^tm1Cxd
• Name: fibroblast growth factor receptor 2; targeted mutation 1, Chu-Xia Deng
• MGI ID: MGI:2153790
• Synonyms: Fgfr2^deltaIgIII, Fgfr2(DeltaIII)
• Gene: Fgfr2 Location: Chr7:129764181-129868538 bp, - strand Genetic Position: Chr7, 73.19 cM
• Alliance: Fgfr2^tm1Cxd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:46380
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced a genomic fragment containing exons 7, 8 and 9 of the gene. These exons encode Ig domain IIIa, b and c. A mutant transcript that splices exon 6 in frame to exon 10 was detected by RT-PCR analysis, although at about one fifth of the wild-type levels. Western blot analysis revealed that a mutant protein is expressed in E10.5 homozygous embryos, but binding assays demonstrated that this mutant protein did not bind ligand. (J:46380)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fgfr2 Mutation: 90 strains or lines available

References

• Original: J:46380 Xu X, et al., Fibroblast growth factor receptor 2 (FGFR2)-mediated reciprocal regulation loop between FGF8 and FGF10 is essential for limb induction. Development. 1998 Feb;125(4):753-65
• All: 6 reference(s)