Fgfr2^tm1Dsn
Targeted Allele Detail

Summary

• Symbol: Fgfr2^tm1Dsn
• Name: fibroblast growth factor receptor 2; targeted mutation 1, Clive Dickson
• MGI ID: MGI:2153810
• Synonyms: Fgfr2(IIIb)^-cond, Fgfr2-IIIb^flox
• Gene: Fgfr2 Location: Chr7:129764181-129868538 bp, - strand Genetic Position: Chr7, 73.19 cM
• Alliance: Fgfr2^tm1Dsn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:59285
• Parent Cell Line: GK129 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: LoxP sites were inserted flanking exon IIIb as follows: A loxP flanked neomycin reistance cassette was inserted in the intron 3' to exon IIIb and an additional loxP site was inserted into the intron 5' to exon IIIb. Correctly targeted ES cells were transiently transfected with a vector expressing Cre recombinase, and lines were selected that retained exon IIIb with flanking loxP sites, and used to inject blastocysts. (J:59285)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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cn1	Fgfr2^tm1Dsn/Fgfr2^tm1Dsn Tg(KRT5-cre)5132Jlj/0	involves: 129P2/OlaHsd * C57BL/6J * DBA/2J

Phenotypes:

Affected Systems	cn1
show or hide all annotated terms
adipose tissue	√
adipose tissue inflammation	√
cellular	√
increased keratinocyte proliferation	√
endocrine/exocrine glands	√
sebaceous gland atrophy	√
growth/size/body	√
epidermal cyst	√
hematopoietic system	√
increased gamma-delta T cell number	√
homeostasis/metabolism	√
increased incidence of tumors by chemical induction	√
immune system	√
adipose tissue inflammation	√
increased gamma-delta T cell number	√
skin inflammation	√
integument	√
increased keratinocyte proliferation	√
sebaceous gland atrophy	√
skin inflammation	√
abnormal zigzag hair morphology	√
abnormal nail morphology	√
abnormal hair follicle morphology	√
abnormal hair follicle orientation	√
abnormal hair cycle	√
abnormal epidermal layer morphology	√
epidermal cyst	√
parakeratosis	√
thick epidermis	√
increased skin papilloma incidence	√
neoplasm	√
increased incidence of tumors by chemical induction	√
increased skin papilloma incidence	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:	27 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fgfr2 Mutation: 90 strains or lines available

References

• Original: J:59285 De Moerlooze L, et al., An important role for the IIIb isoform of fibroblast growth factor receptor 2 (FGFR2) in mesenchymal-epithelial signalling during mouse organogenesis. Development. 2000 Feb;127(3):483-92
• All: 14 reference(s)