Ap1q^C57BL/6J
QTL Variant Detail

Summary

• QTL variant: Ap1q^C57BL/6J
• Name: alcohol preference 1 QTL; C57BL/6J
• MGI ID: MGI:2153885
• QTL: Ap1q Location: Chr1:146908199-167586222 bp Genetic Position: Chr1, cM position of peak correlated region/allele: 63.28 cM
• QTL Note: genome coordinates based on the boundaries of the QTL region

Variant origin

• Strain of Specimen: C57BL/6J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers increased alcohol preference compared to DBA/2J. (J:49707)
• Inheritance: Dominant

Notes

Candidate Genes

J:98595

Microarray analysis was used to identify genes showing alcohol-induced differential expression between inbred strains C57BL/6J and DBA/2J. These genes were then correlated to alcohol-related QTLs to identify potential candidate genes. The experiment involved identifying genes expressed differentially in alcohol-induced and non-induced animals but the following list of candidate genes are only for alcohol-induced states.

On mouse Chromosome 1, several candidate genes were identified for Ap1q at 60 cM andAlcw1 at 95.8 cM. Candidate genes for Ap1q are Akp3 (51.7 cM), Gbx2 (65 cM), and Pam (57.5 cM). Candidate genes for Alcw1 are Adss, 2810484M10Rik, and Mark1.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:49707

Genome scan was performed on 218 (C57BL/6J x DBA/2J)F2 intercross animals using 101 microsatellite markers to identify QTLs associated with alcohol preference. Parental strain C57BL/6J exhibits higher alcohol preference compared to parental strain DBA/2J. Significant alcohol preference QTLs mapped to mouse Chromosome 1 spanning 45 cM - 95 cM (Ap1q, LOD = 4.5 between D1Mit194 and D1Mit110), mouse Chromosome 4 spanning 65 cM - telomere (Ap3q, LOD = 4.7 at D4Mit42), and mouse Chromosome 9 spanning 10 cM - 35 cM (Apq5, LOD = 4.8 cM at D9Mit330). Suggestive QTLs mapped to mouse Chromosome 2 spanning 30 cM - 50 cM (Ap2q, LOD = 3.1 at D2Mit61), mouse Chromosome 10 (Ap4q) spanning 18 cM - 57 cM, and mouse Chromosome 3 spanning 38 cM - 60 cM (Ap6q, LOD = 3.4 between D3Mit12 and D3Mit86). Ap1q, Ap2q, Ap3q, and Ap4q were provisionally mapped in BXD RI strains and confirmed in the present study, while Ap5q and Ap6q are novel. C57BL/6J-derived alleles increases alcohol preference at all loci mapped. Ap1q is follows adominant mode of inheritance and maps near Chrnd (Acrd), Chrng (Acrg), and Htr5b. Ap2q is consistent with a recessive or additive mode of inheritance and maps near Scn1a, Scn2a, Scn3a, and Scn7a . Ap3q is consistent with a dominant or additive mode of inheritance and maps near Htr1da, Htr1db, Ahd1 (Ssdh1), and Tas1r3 (Sac). Ap5q is consistent with a dominant or additive mode of inheritance and maps near Htr1b and Drd2. Ap6q is most consistent with an additive mode of inheritance and maps near the alcohol dehydrogenase gene complex at approximately 71.2 cM on mouse Chromosome 3.

References

• Original: J:49707 Tarantino LM, et al., Confirmation of quantitative trait loci for alcohol preference in mice. Alcohol Clin Exp Res. 1998 Aug;22(5):1099-105
• All: 1 reference(s)