Fatq1^MH
QTL Variant Detail

Summary

• QTL variant: Fatq1^MH
• Name: percent fat, subcutaneous and gonadal, QTL 1; MH
• MGI ID: MGI:2154089
• QTL: Fatq1 Location: unknown Genetic Position: Chr1, Syntenic

Variant origin

• Strain of Specimen: MH line

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers increased adiposity compared to C57BL/6J. (J:55483)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:55483

Authors used a composite base population from four outbred strains (NIH, ICR, CF-1 and CFW) to select for lines showing a difference in heat loss. After 15 generations of selection, the MH line showed higher heat loss, consumed more feed, were leaner andmore active than the ML line of mice. C57BL/6J was chosen as the inbred line to create a resource population from a cross between a selection line and an inbred line. Average heat loss and feed intake of the C57BL/6J mice were similar to that of mice from the ML line but their body composition was similar to the MH line. To identify QTLs that are responsible for heat loss and other phenotypes related to energy balance, a complete genome wide scan was conducted in a (MH x C57BL/6J)F2 intercross (n = 560).Regions harboring QTL with the greatest effects were then evaluated in a (MH x ML)F2 intercross (n = 560). A QTL influencing gonadal fat and combined (subcutaneous and gonadal) fat, Fatq1 (LOD = 7.95 for gonadal fat and 7.36 for combined fat, 5.9 and 5.4%0f the respected residual variance explained) was identified with a peak at 61 cM on Chromosome 1 in the (MH x C57BL/6J)F2 intercross. The MH allele was responsible for increased fatness for this QTL.

J:152067

To determine QTLs effecting body weight C57BL/6J mice were mated to PWK/PhJ and the resulting F2 hybrids were used in mapping. A total of 479 F2 mice (235 female and 244 male) were bred from two types of reciprocal crosses: 236 and 243 F2 mice were obtained from (B6 female X PWK male) F1 Female X (B6 female X PWK male) F1 male crosses and (PWK female X B6 male) F1 female with (B6 female X PWK male) F1 male crosses respectively.

Four phenotypes were analyzed, these were body weight, lean weight,fat weight, and percent fat (fat weight/body weight X 100). C57BL/6J mice are heavier then PWK/PhJ mice.

The following new and existing QTLs were mapped

(following the QTL is the linked peak marker with cM locations):

Fatq1(D1Mit440 - 54 cM),

Pbwg1.1(D2Mit285 - 86 cM),

Adip22 (was identified as Adip10)(D2Mit293 -11cM),

Gnf5 (D2Mit285 - 86 cM),

Wt10q4 (was identified as Wt10q2) (D3Mit57 - 55 cM),

Btwq13 (D3Mit5 - 25cM),

Afw13 (was identified as Afw1) (D3Mit5 - 25 cM),

Afpq8 (was identified as Afpq1)(D3Mit256 - 66cM),

Bw6b (D3Mit17 - 32 cM),

Epb4.1 (D4Mit17 - 32 cM),

Adip23 (was identified as Adip11)Adip11 (D4Mit17 -32 cM),

Bwq8 (D5Mit123- 3cM),

Bwtq14 (was identified as Bw6d and Bwob) (rs3719351 - 49 cM),

Afw3 (D5Mit314 - 59 cM),

Bwtq7 (was identified as Bwtq10)(D6Mit198 - 67 cM),

Pfat5 (D6Mit284 - 38 cM),

Pbwg3(D7Mit223 - 72 cM),

Mtbcq9 (was identified as Mtbcq8) (D9Mit129 - 26 cM),

Adip21 (was identified as Adip5)(D9Mit123 - 42 cM),

Adip24 (was identified as Adip15)(D10Mit96 - 56 cM),

Bw4(D11Mit285 -48 cM),

Adip19 (D11Mit285 - 52 cM),

Fatpad3 ( D13Mit144 - 48 cM),

Bw40 (was identified as Bw10) (D13Mit144 - 48 cM),

Fatq1 (D16Mit107 - 3 cM)'

and Adip25 (was identified as Adip17) (D16Mit107 - 3 cM).

The new QTLs mapped in this study were:

Bwtq13,

Bwq13 (alias Bwq8),

Bwq9 (alias Pbwq1.1),

Bwq11 (alias Pbwq3),

Pfat5 (was identified as Pfat2),

Gnf5 (was identified as Gnf3)

and Adip19.

10.03.2014 Curator Note:

We had originally associated the abdominal fatweight QTL identified here mapping to (D3Mit5- 25 cM) on mouse Chr 3, as Afw1. Afw1 was originally mapped using an outbred cross of DU6 xDUK, in J:49578 in 1998. We consider the current mapping study a separate mapping experiment and have named the QTL identified here as Afw13.

01.07.2015 Curator Note:

We had originally associated the QTL identified here, mapping to D13Mit144 - 48 cM as Bw10. However, Bw10 was originally mapped using a different cross, an outbred cross of DU6 x DUK, in J:49578, in 1998. We consider the current mapping study a separate mapping experiment and have named the QTL identified here as Bw40.

01.23.2015 Curator Note:

Because Adip5 was origniallymapped in J:62610 in 2001 using an (LG/J x SM/J) intercross, which differs from the cross used here, we consider the current study a separate mapping experiment and have named this QTL Adip21.

Adip10, Adip11, Adip15 and Adip17 mentioned here were all originally mapped in J:103841 in 2006 using an (SM/J x NZB/BlNJ)F2 intercross, which also differs from the cross used here. For thesame reasons noted above we have named these markers Adip22, Adip23, Adip24 and Adip25 respectively.

03.11.2015 Curator Note:

Because Bwtq10 was originally mapped in J:103841 in 2006 using (SM/J x NZB/BlNJ)F2 intercross, which differs from the cross used here, we consider the current study a separate map experiment and have named this QTL Bwqt7, (D6Mit198- 67 cM).

04.2.2015 Curator Note:

Mtbcq8 was originally mapped in J:93070 in 2004 using an (NMRI8/Fbn x DBA/2OlaHsd)F2 intercross, which differs fromthe cross used here, we consider the current study a new map experiment and have named the QTL Mtbcq9 (D9Mit129 - 26cM).

04.14.2015 Curator Note:

Because the abdominal fat percent QTL 1, (Afpq1) was orginally mapped in J:49578 in 1998 using an an outbredcross of DU6 xDUK, which differs from the cross used here we consider the current mapping study a separate mapping experiment and have named the QTL Afpq8.

04.15.2015 Curator Note:

Because Wt10q2 was originally mapped in J:55483 in 1999 using an (MHxC57BL/6J)F2 intercross, which differs from the cross used here, we consider the current mapping study a separate mapping experiment and have named the QTL Wt10q4.

06.16.2015 Curator Note:

Pbwq1.1 was orginally mapped in J:121981 using B6.Cg congenic mice, which differ from the C57BL/6J X PWK/PhJ mice used here. We consider the current mapping study a separate mapping experiment and have named this QTL, mapping to Chr 2 with D2Mit285, Bwq9.

Pbwq3 was originally mapped in J:88536 using an (C57BL/6J x M. m. castaneus)F1 x M. m. castaneus backcross, which also differs from the cross used here. We have named this QTL, mapping to Chr 7 with D7Mit223, Bwq11.

References

• Original: J:55483 Moody DE, et al., Identification of quantitative trait loci influencing traits related to energy balance in selection and inbred lines of mice. Genetics. 1999 Jun;152(2):699-711
• All: 1 reference(s)