Gvhd1^C57BL/10J
QTL Variant Detail

Summary

• QTL variant: Gvhd1^C57BL/10J
• Name: graft-versus-host disease 1; C57BL/10J
• MGI ID: MGI:2154093
• QTL: Gvhd1 Location: Chr1:174456299-174456436 bp Genetic Position: Chr1, cM position of peak correlated region/allele: 81.08 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: C57BL/10J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• Mutation details: In donor animals, this allele confers increased graft versus host disease severity in recipient animals compared to DBA/2J. (J:52834)
• Inheritance: Recessive

Notes

Animals homozygous for C57BL/10J-derived alleles at both Gvhd1 and Gvhd2 induced the most severe disease response in F1 recipients.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:52834

64 polymorphic markers were typed in a population of (B10.D2-H2^d x DBA/2J)F1 x B10.D2-H2^d backcross mice and (B10.D2-H2^d x DBA/2J)F1 x DBA/2J backcross mice to identify QTLs associated with graft-versus-host disease (B10=C57BL/10J; D2=DBA/2J). Spleen cell from backcross animals were intraperitoneally injected into (C57BL/10J x DBA/2J)F1 recipients to induce graft-versus-host disease, and splenomegaly was measured as an indicator of disease. F1 animals receiving spleen cells from (B10.D2-H2^d x DBA/2J)F1 x B10.D2-H2^d backcross donors showed higher incidence of graft-versus-host disease than F1 animals receiving spleen cells from (B10.D2-H2^d x DBA/2J)F1 x DBA/2J backcross donors. Donor animals homozygous for C57BL/10J-derived alleles appear to induce more severe disease in F1 recipients. QTL analysis revealed a major locus on distal mouse chromosome 1 segregating with this trait. This locus, Gvhd1 (graft-versus-host disease 1), maps to 78 cM - 112 cM on mouse chromosome 1 yielding a peak LOD score = 27 at D1Mit150. Candidate genes near the region of Gvhd1 are Cd48 and Mtv7. A second locus, Gvhd2, mapped to approximately 76 cM - 81 cM on mouse chromosome 4 yielding a peak LOD score = 7.2 near D4Mit42. A candidate gene in the region of Gvhd2 is Tnfrsf8 (Cd30). Animals homozygous for C57BL/10J-derived alleles at both Gvhd1 and Gvhd2 induced the most severe disease response in F1 recipients. A suggestive locus mapped to mouse chromosome 6 near D6Mit86 (P<0.02).

References

• Original: J:52834 Allen RD, et al., Genetics of graft-versus-host disease, I. A locus on chromosome 1 influences development of acute graft-versus-host disease in a major histocompatibility complex mismatched murine model. Immunology. 1999 Feb;96(2):254-61
• All: 1 reference(s)