Hpic2^DBA/2J
QTL Variant Detail

Summary

• QTL variant: Hpic2^DBA/2J
• Name: haloperidol induced catalepsy 2; DBA/2J
• MGI ID: MGI:2154104
• QTL: Hpic2 Location: Chr1:175468817-181400659 bp Genetic Position: Chr1, cM position of peak correlated region/allele: 81.08 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: DBA/2J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers susceptibility to haloperidol-induced catalepsy. (J:61419)
• Inheritance: Not Specified

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:68417

The authors use a novel approach in mapping QTLs involved in murine behavioral phenotypes. A genome wide scan was performed with (C57BL/6J x DBA/2J)F2 and (BALB/cJ x LP/J)F2 intercrosses crosses to map QTLs affecting in three different behavioral phenotypes. The strains chosen have widely differing polymorphisms and phenotypes, and the QTLs identified from each cross do not appear to have overlap. Significant QTLs (LOD>4.3 threshold) for basal locomotor activity (Bslm3), haloperidol-induced catalepsy (Hpic2), and ethanol-induced locomotion (Etlm1) were mapped to Chromosome 1 at positions 55 - 85 cM, 70 - 100 cM, and 70 - 100 cM, respectively. Etlm1 and Hpic2 were identified from the (C57BL/6J x DBA/2J)F2 cross. Bslm3 was identified from the (BALB/cJ x LP/J)F2 cross. The authors also used a subtractive method to resolve a broad QTL (60 - 100 cM) for basal activity on distal Chromosome 1. Genotyping information from (C57BL/6J x DBA/2J)F2, (C57BL/6J x A/J)F2, and (C57BL/6J x BALB/cJ)F2 intercrosses was used to screen 254 microsatellite markers across the locus. Only markers polymorphic between all four strains (35 markers) were further analyzed. These highly informative markers revealed 2 separate QTLs: one previously reported by Talbot for basal activity at 67 cM and another at 95 - 105 cM.

J:61419

678 male animals from a (C57BL/6J x DBA/2J)F2 intercross were used to map catalepsy-related QTLs. Markers were typed at an average density of 20 cM. Hpic2, a QTL for haloperidol-induced catalepsy, mapped to 100 cM on Chromosome 1 with a LOD score of 5.0 at D1Mit150. Hpic2 maps to the same relative position as a QTL for open field activity identified by Flint (1995) and Gershenfeld (1997). A significant genetic association exists for catalepsy and open field activity (Kline, 1998). No obvious candidate genes were found in this region. The dilute coat color was found to segregate against the parental phenotype. Dilute DBA/2J animals have a haloperidol ED50 of 0.4 mg/kg and dilute C57BL/6J animals have a haloperidol ED50 of 4.0 mg/kg.

References

• Original: J:61419 Patel NV, et al., Detection and mapping of quantitative trait loci for haloperidol-induced catalepsy in a C57BL/6J x DBA/2J F2 intercross. Behav Genet. 1999 Sep;29(5):303-10
• All: 3 reference(s)