Obq2^129T2/SvEmsJ
QTL Variant Detail

Summary

• QTL variant: Obq2^129T2/SvEmsJ
• Name: obesity QTL 2; 129T2/SvEmsJ
• MGI ID: MGI:2154250
• QTL: Obq2 Location: Chr1:20941620-20941887 bp Genetic Position: Chr1, cM position of peak correlated region/allele: 6.5 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: 129T2/SvEmsJ

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers increased adiposity compared to EL/Suz. (J:33858)
• Inheritance: Dominant

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Notes

Candidate Genes

J:99477

Authors used novel data mining tool ExQuest to identify novel candidate genes for existing diabesity QTLs. Next, candidate gene expression in the liver, adipose, and pancreas of diabesity-prone Tally Ho mice and diabesity-resistant C57BL/6J mice was assessed by quantitative PCR analysis. Several potential candidate genes, some with no previous association to diabesity QTLs, were identified. Since QTL intervals may be large and could contain hundreds or thousands of potential candidate genes, this method allows researchers to focus on those with strong potential as well as identify novel candidate genes.

A potential candidate gene for Obq2 at 15 cM on mouse Chromosome 1 as identified by ExQuest is Gsta3. For QTLs Obq7 (28.7 cM), Wt6q1 (27 cM), Insq2 (36cM), and Insq6 (37 cM), potential candidate genes Aox1 (23.2 cM), Fn1 (36.1 cM), Pecr, Igfbp2 (36.1 cM), Plcd4 (39.2 cM), Scg2 (43.6 cM), Irs1, and Inpp5d (57 cM) were identified. For QTL Nidd6 (77 cM), potential candidate gene Qscn6 was identified. For QTL Obq9 (88.4 cM), potential candidate genes Fmo1, Fmo3, and Apoa2 (92.6 cM) were identified. For QTL Wt6q2 (108 cM), potential candidate gene Hsd11b1 was identified.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:33858

Loci affecting obesity were mapped in a (129T2/SvEmsJ x EL/Suz)F2 intercross population using QTL analysis and DNA pooling strategy. Parental strain 129T2/SvEmsJ exhibits a lean phenotype whereas parental strain EL/Suz is prone to obesity. Significant linkage to adiposity index (LOD=8.0), percent inguinal fat (LOD=6.1), percent gonadal fat (LOD=8.4), percent retroperitoneal fat (LOD=7.9), body mass index (LOD=5.1), and 16 week body weight (LOD=4.5) was detected at a locus spanning 21 cM - 49 cM on mouse Chromosome 7. This locus was named Obq1 with peak linkage near D7Mit248. EL/Suz-derived alleles confer increased adiposity in a dominant fashion at Obq1. A second locus, Obq2, was detected at 12 cM - 15 cM on mouse Chromosome 1. Obq2 is linked to adiposity index (LOD=4.0) at D1Mit231. 129T2/SvEmsJ-derived alleles confer increased adiposity in a dominant fashion at Obq2.

References

• Original: J:33858 Taylor BA, et al., Detection of obesity QTLs on mouse chromosomes 1 and 7 by selective DNA pooling. Genomics. 1996 Jun 15;34(3):389-98
• All: 1 reference(s)