Pbw1^DBA/2J
QTL Variant Detail

Summary

• QTL variant: Pbw1^DBA/2J
• Name: pentobarbital withdrawal QTL 1; DBA/2J
• MGI ID: MGI:2154307
• QTL: Pbw1 Location: Chr1:172831765-172831892 bp Genetic Position: Chr1, cM position of peak correlated region/allele: 80.33 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: DBA/2J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers increased pentobarbital withdrawal severity compared to C57BL/6J. (J:54502)
• Inheritance: Recessive

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:54502

Authors used mice that are resistant versus sensitive to pentobarbital withdrawal to identify QTLs associated with risk for physiological dependence on barbiturates. QTL analysis with the BXD recombinant inbred strains for pentobarbital withdrawal severity identified markers associated with withdrawal located between 73 and 98.7 cM from the centromere. This locus was confirmed using a B6D2 F2 cross and the selectively bred High Pentobarbital Withdrawal (HPW) and Low Pentobarbital Withdrawal (LPW) lines. In the F2 cross, withdrawal severity was strongly associated with genotypic status at D1Mit206 (P = 0.00003). Mice homozygous for the DBA/2J allele at D1Mit206 show more severe withdrawal than C57BL/6J homozygotes or heterozygotes. Maximum likelihood analysis of the F2 data indicated a maximum LOD of 3.8 (df = 1) at the D1Mit206 locus. Differences in withdrawal severity between the HDW and LDW lines were highly correlated with differences in allele frequencies at D1Mit206 [P = 3.6 x 10 e(-8), LOD = 6.6, df = 1]. This QTL accounts for ~5% of phenotypic variance in withdrawal severity among D2- and B6- derived mice, representing 25% of the genetic variance.

References

• Original: J:54502 Buck K, et al., Quantitative trait loci affecting risk for pentobarbital withdrawal map near alcohol withdrawal loci on mouse chromosomes 1, 4, and 11. Mamm Genome. 1999 May;10(5):431-7
• All: 1 reference(s)