Tir6a^A/JOlaHsd
QTL Variant Detail

Summary

• QTL variant: Tir6a^A/JOlaHsd
• Name: trypanosome infection response 6a; A/JOlaHsd
• MGI ID: MGI:2154350
• QTL: Tir6a Location: unknown Genetic Position: Chr1, Syntenic

Variant origin

• Strain of Specimen: A/JOlaHsd

Variant description

• Allele Type: QTL
• Inheritance: Not Specified

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:63779

A further refined mapping for loci responsible for trypanosomiasis resistance in mice was achieved using advanced intercross lines. Advanced intercross lines were constructed by breeding the progeny from resistant C57BL/6JOlaHsd mice with susceptible mice from both the A/JOlaHsd and the BALBc/OlaHsd strains respectively. Phenotyping, genotyping, and linkage analysis was performed on the G6 progeny designated (B/B6)G6 and (A/B6)G6.

Table 1:

Chromosome 17

In the BALBc/OlaHsd x C57BL/6JOlaHsd G6, (B/B6)G6, population of 892 mice a previoulsy indentified QTL, Tir1 [J:40771], mapped to mouse Chromosome 17 at 18.2 cM nearest marker D17Mit16 (LOD 20.08). The 95% confidence interval was refined to 2.8 cM.

In the A/JOlaHsd x C57BL/6JOlaHsd G6, (A/B6)G6, population of 1986 mice a previoulsy identified QTL, Tir4 [J:40771] mapped to 17.9 cM nearest marker D17Mit175, (LOD 19.35). The 95% confidence interval was refined to 1.3 cM.

Analysis of the combined G6 populations provided an association nearest marker D17Mit16 (LOD 37.56). The 95% confidence interval in the combined data was refined further to 0.9 cM.

Chromosome 5

On mouse Chromosome 5 in the A/JOlaHsd x C57BL/6JOlaHsd G6 population a previously identified QTL, Tir5 [J:40771], mappped to 42.0 cM nearest marker D5Mit113 (LOD 4.63). The 95% confidence interval spanned 16 cM.

In the BALBc/OlaHsd x C57BL/6JOlaHsd G6 population, the previously identified Tir2 QTL identified on Chromosome 5 using the same strains [J:40771] did not acheive significance.

When the data from both Chromosome 5 G6 poulations was combined a peak was detected near D5Mit114 with a LOD score of 5.46 and a 95% confidence interval spanning 12 cM.

Chromosome 1

The Tir3 QTL previoulsy identified in J:98907 using an F2 cross, of the same strains used here, was resolved into 3 distinct QTL in the BALBc/OlaHsd x C57BL/6JOlaHsd G6 population.

Tir3a mapped to 60.0 cM nearest marker D1Mit87 with a LOD score of 4.15 and a 95% confidence interval spanning 9cM.

Tir3b mapped to 74.0 cM nearest markers D1Mit286 and D1Mit268 with a LOD score of 5.88 and a 95% confidence interval spanning 11 cM.

Tir3c mapped to 92.0 cM nearest marker D1Mit113 with a LOD score of 3.78 and a 95% confidence interval spanning 9.0 cM.

5.28.2015 Curators Note - The 3distinct QTL mapped on Chromosome 1 using the A/JOlaHsd x C57BL/6JOlaHsd G6 population represent 3 unique QTL not previously mapped using these strains. We consider this a separate mapping experiment and have named the larger QTL region Tir6 spanning a region for 62.5-96.0 cM encompassing Tir6a, Tir6b and Tir6c [Table 1.].

Tir6a mapped to 62.5 cM nearest marker D1Nds2 with a LOD score of 8.07 and a 95% confidence interval of 0.7 cM.

Tir6b mapped to 70.0 cM nearest markers D1Mit286 and D1Mit102 with a LODscore of 6.8 and a 95% confidence interval spanning 16 cM.

Tir6c mapped to 96.0cM nearest D1Mit16 with a LOD score of 5.49 and a 95% confidence interval spanning 18 cM.

Combining the data from the differing G6 populations for Tir3a and Tir6a resulted ina LOD score of 9.94 nearest D1Nds2 with a 95% confidence interval of 1.8 cM.

Combining the data from the differing G6 populations for Tir3b and Tir6b resulted in a LODscore of 11.3 nearest markers D1Mit286 and D1Mit102 with a 95% confidence interval spanning 10 cM.

Combining the data from the differing G6 populations for Tir3c and Tir6c resulted in a LOD score of 7.62 nearest D1Mit113 with a 95% confidence interval of 8 cM.

J:161792

A further refined mapping for loci responsible for trypanosomiasis resistance in mice was achieved using advanced intercross lines 12 (A12). [Table 1.] An F12 population of AILs, A/J(susceptible) x C57BL/6J (resistant) mice fixed for the susceptible allele at Tir1 were informative in refining and redefining the mapping of Tir loci.

In the F12 population Tir1 was not mapped as its location was well defined in F2 and F6 populations mapping to the same general region of mouse Chr 17, (17.9 cM).

Using F12 mice Tir2 showed a peak ( LOD 3.81) at D5Mit258.

Tir3a, Tir3b and Tir3c all mapped to mouse Chromosome 1 at positions 67 cM(LOD 4.49), 76 cM (LOD 3.48), and 86 cM(LOD 3.18) respectively.

6.9.2015 Curator Note: Tir2 was orginally mapped using a BALB/cJOlaHsd[ce] x C57BL/6JOlaHsd (BXB6) cross in J:98907 in 1996. Tir3a, Tir3b and Tir3c were all mapped using the same cross in J: 63779 in 2000. Because the F12 mice used here were generated from different strains, A/J X C57BL/6J, we have equated the QTL identified here with previoulsy mapped QTL using the same cross also done in J:63779 in 2000. These QTL have been associated with Tir5, Tir6a, Tir6b and Tir6c repectively.

References

• Original: J:63779 Iraqi F, et al., Fine mapping of trypanosomiasis resistance loci in murine advanced intercross lines. Mamm Genome. 2000 Aug;11(8):645-8
• All: 2 reference(s)