Sle1c^C57BL/6J
QTL Variant Detail

Summary

• QTL variant: Sle1c^C57BL/6J
• Name: systematic lupus erythematosus susceptibility 1c; C57BL/6J
• MGI ID: MGI:2154455
• QTL: Sle1c Location: Chr1:188594514-189303617 bp Genetic Position: Chr1, cM position of peak correlated region/allele: 94.73 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: C57BL/6J

Variant description

• Allele Type: QTL
• Inheritance: Recessive

Notes

Candidate Genes

J:73128

The NZM2410 interval contains the Sle1 QTLs and originated from an (NZB x NZW) cross. In order to construct congenic strains of mice which contain selected Sle loci, NZM2410 were backcrossed to C57BL/6J mice six successive times, screening progeny withPCR or SSR markers for specific genomic intervals. One such locus Sle1c resided in an interval of mouse Chromosome 1 that contained Cr2 which is a possible candidate for Sle1c. There were at least 16 nucleotide sequence differences between NZW and C57BL/6J at the Cr2 locus. The authors provided evidence suggesting that Cr2 is the disease susceptibility gene corresponding to the Sle1c QTL.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:67544

Systemic lupus erythematosus (Sle) is measured via anti-chromatin IgG production. Fine mapping of loci around Sle1 indicate that Sle1 actually corresponds to a cluster of loci related in part to Sle1. Various mating paradigms were used to dissect the Sle1 interval, including 493 (NZM2410 X C57BL/6J) F1 meioses. In addition (B6.Sle1 X C57BL/6J) x C57BL/6J recombinants were intercrossed to create more recombinants and to develop sublines on a C57BL/6J background.

Interestingly three non overlapping regions were identified within Sle1 designated as Sle1a, Sle1b and Sle1c. The following congenic intervals were identified.

Sle1a is included in an interval bounded by D1Mit15 and D1Mit353.

Sle1b is included in an interval bounded by D1Mit113 and D1Mit407 and by D1Mit113 and D1Mit206.

Sle1c is included in an interval bounded by D1Mit274 and D1Mit17.

References

• Original: J:67544 Morel L, et al., The major murine systemic lupus erythematosus susceptibility locus, Sle1, is a cluster of functionally related genes. Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1787-92
• All: 3 reference(s)