Cdk4^tm1Bbd
Targeted Allele Detail

Summary

• Symbol: Cdk4^tm1Bbd
• Name: cyclin dependent kinase 4; targeted mutation 1, Mariano Barbacid
• MGI ID: MGI:2154520
• Synonyms: Cdk4-, Cdk4ⁿ, Cdk4^nc, Cdk4^neo
• Gene: Cdk4 Location: Chr10:126899404-126903157 bp, + strand Genetic Position: Chr10, 74.5 cM, cytoband D3
• Alliance: Cdk4^tm1Bbd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:54534
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A loxP-flanked neomycin resistance cassette was inserted between exons 1 and 2. In addition, the AAGCCCGA sequence in exon 2 (nt 81-88) was replaced by AAGCTTGT. This alters codon 24 from one corresponding to arginine to a cysteine. A third silent substitution at position nt85 was used to create a diagnostic restriction enzyme site to monitor the presence of the mutation. RT-PCR analysis revealed that no transcript was produced from this allele in testis, ovaries or pancreas. Western blot analysis demonstrated that no protein was detectable in fibroblasts isolated from E14.5 homozygous embryos. (J:54534)

Disease models

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cdk4 Mutation: 58 strains or lines available

References

• Original: J:54534 Rane SG, et al., Loss of Cdk4 expression causes insulin-deficient diabetes and Cdk4 activation results in beta-islet cell hyperplasia. Nat Genet. 1999 May;22(1):44-52
• All: 20 reference(s)