Itgb1^tm1Son
Targeted Allele Detail

Summary

• Symbol: Itgb1^tm1Son
• Name: integrin beta 1 (fibronectin receptor beta); targeted mutation 1, Arnoud Sonnenberg
• MGI ID: MGI:2154543
• Synonyms: beta1D ko
• Gene: Itgb1 Location: Chr8:129412135-129459681 bp, + strand Genetic Position: Chr8, 76.09 cM, cytoband E2
• Alliance: Itgb1^tm1Son page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:47279
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP-flanked neomycin resistance cassette replaced exon D, which encodes sequences for the beta1D alternative isoform. The neomycin cassette was deleted by Cre-mediated recombination in ES cells prior to blastocyst injection, leaving a single loxP site instead of exon D. Northern blot analysis revealed that no beta1D specific transcript was present, but transcripts of other splice variants of the gene were not afftected by this mutation. Western blot analysis confirmed the absence of the beta1D specific protein. (J:47279)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Itgb1 Mutation: 60 strains or lines available

References

• Original: J:47279 Baudoin C, et al., Knockout and knockin of the beta1 exon D define distinct roles for integrin splice variants in heart function and embryonic development. Genes Dev. 1998 Apr 15;12(8):1202-16
• All: 1 reference(s)