Bglu1^C3H/HeSlc
QTL Variant Detail

Summary

• QTL variant: Bglu1^C3H/HeSlc
• Name: blood glucose level 1; C3H/HeSlc
• MGI ID: MGI:2154950
• QTL: Bglu1 Location: Chr2:117942930-174324863 bp Genetic Position: Chr2, cM position of peak correlated region/allele: 59.34 cM
• QTL Note: genome coordinates based on the boundaries of the QTL region

Variant origin

• Strain of Specimen: C3H/HeSlc

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers decreased blood glucose levels compared to C57BL/6Slc.
• Inheritance: Recessive

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:62933

Inbred strain C57BL/6Scl secretes less insulin and exhibits higher blood glucose levels than inbred strain C3H/HeSlc when subject to an intraperitoneal glucose tolerance test (IPGTT). A pooling strategy was used to identify QTLs associated with glucose intolerance in a population of (C3H/HeSlc x C57BL/6Slc)F2 male animals. 30 F2 males with the highest 30-minute glucose values and 30 F2 males with the lowest 30-minute glucose values were typed for 87 polymorphic markers spanning the 19 autosomes. Significant loci were confirmed by screening (using the same set of markers) a population of 59 F3 male animals generated from crossing F2 animals with the highest (high x high) and lowest (low x low) glucose values. A locus on mouse Chromosome 2, Bglu1, is significantly associated with blood glucose levels. Bglu1 gave a LOD score of 8.3 at D2Mit48 and has a QTL range from 65 cM - 105 cM defined by D2Mit62 and D2Mit265. C57BL/6Slc-derived alleles appear to have a dominant effect in increasing blood glucose levelsat Bglu1. Candidate genes in the region of Bglu1 include Chgb, Pcsk2, A (agouti), Kcnb1, and Hnf4a. A second locus associated with blood glucose levels, Bglu2, mapped to mouse Chromosome 13 giving a LOD score of 4.2 at D13Mit148 with a QTL range from 59 cM- 61 cM defined by D13Mit148 and D13Mit76. C57BL/6Slc-derived alleles appear to influence blood glucose levels in a codominant fashion at Bglu2. Candidate genes in the region of Bglu2 include Pcsk1 and Pik3r1. Direct sequencing of Pcsk1 and Pcsk2 did notreveal polymorphisms or mutations in C57BL/6Scl or C3H/HeSlc parental inbred strains.

References

• Original: J:62933 Kayo T, et al., Identification of two chromosomal loci determining glucose intolerance in a C57BL/6 mouse strain. Comp Med. 2000 Jun;50(3):296-302
• All: 1 reference(s)