Nidd5^TSOD
QTL Variant Detail

Summary

• QTL variant: Nidd5^TSOD
• Name: non-insulin-dependent diabetes mellitus 5; TSOD
• MGI ID: MGI:2154986
• Synonyms: TSOD
• QTL: Nidd5 Location: unknown Genetic Position: Chr2, cM position of peak correlated region/allele: 31.66 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: TSOD

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers increased body weight and decreased insulin levels compared to BALB/cA.
• Inheritance: Other (see notes)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Notes

This allele is inherited in a dominant fashion for insulin levels and inherited additively for body weight.

Candidate Genes

J:91835

The correlation between gene expression and dietary interaction was examined in a population of (BALB/cStCrlfC3H/Nctr x VY/WffC3Hf/Nctr-A(vy)/a)F1 animals. A/a and A/A^vy animals were placed on a 70% (calorie restricted) or 100% (non-restricted) diet andliver mRNA levels were assessed for over 18,000 genes using DNA microarrays. Twenty-eight known genes showing statistically significant differential expression between the 70% and 100% calorie diets and A/a and A/A^vy genotypes mapped near known diabesity QTLs. These genes may be considered further for candidate genes.

On mouse Chromosome 2, Nek6 showed differential expression between A/a and A/A^vy animals on the restricted diet. Diabesity QTL Nidd5 (34.5 cM) maps near this candidate gene.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:55285

TSOD male mice exhibit both obesity and diabetes enabling that strain to be used in a cross with BALB/cAJcl to elucidate the genetic basis for the phenotype. (BALB/cAJcl x TSOD)F1s and (TSOD x BALB/cAJcl)F1s were intercrossed to provide F2 populations for genotyping using SSLP markers.

A major gene on mouse Chromosome 2 effecting insulin levels and body weight (marker Nidd5) was significantly associated with a region close to markers D2Mit156 and D2Mit91 with a LOD score of 7.95 for insulin and 5.91 for body weight.

References

• Original: J:55285 Hirayama I, et al., Genetic analysis of obese diabetes in the TSOD mouse. Diabetes. 1999 May;48(5):1183-91
• All: 5 reference(s)