Cia2^DBA/1J
QTL Variant Detail

Summary

• QTL variant: Cia2^DBA/1J
• Name: collagen induced arthritis QTL 2; DBA/1J
• MGI ID: MGI:2155050
• QTL: Cia2 Location: Chr2:60520612-60520756 bp Genetic Position: Chr2, cM position of peak correlated region/allele: 34.9 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: DBA/1J

Variant description

• Allele Type: QTL
• Inheritance: Other (see notes)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Notes

Cia2 exhibits additive inheritance.

Candidate Genes

J:81372

Microarray gene expression analysis was used to identify candidate genes for QTLs associated with collagen-induced arthritis. RNA from inflamed paws of collagen-treated mice were used in the microarray analysis and compared to RNA from normal paws. 223 genes showed a four-fold or greater expression difference between inflamed and normal paws. Nine of these genes are located near previously identified arthritis severity/susceptibility QTLs and may be considered for candidates.

For Cia1 on mouse Chromosome 17 (18.6 cM near the H2 locus) differential expression was observed for H2-Eb1 (18.66 cM) and H2-T23 (19.73 cM).

For Cia2 and Cia4 on mouse Chromosome 2 (both are located at 34 cM) differential expression was observed for Fap (36 cM) and Gcg (36 cM).

For Cia3 (48.7 cM) and Cia6 (30 cM) on mouse Chromosome 6 differential expression was observed for Raf1 (52.5 cM) and Slc6a6 (38.2 cM), respectively.

For Cia5 (40 cM) on mouse Chromosome 4 differential expression was observed for Ctsk (47.9 cM) and Cd53(50.5 cM).

For Cia8 (40.4 cM) on mouse Chromosome 10 differential expression was observed for Bsg (42.4 cM).

Mapping and Phenotype information for this QTL, its variants and associated markers

J:53356

The authors performed experiments to localize susceptibility loci for collagen induced arthritis (CIA). F2 crosses between the highly susceptible DBA/1J strain and the highly resistant SWR/J strains were analyzed for the presence of QTLs. A locus, Cia2 on mouse Chromosome 2 had significant evidence of linkage to D2Mit61 (lod score of 6.34). Two-point analysis using BYMARKER showed that the incidence and severity of the CIA phenotype was significantly associated with D2Mit61 (F = 10.5, P<0.0001). Another QTL associated with the age of onset of CIA, Cia4 was also linked to D2Mit61 (lod score of 3.2, F = 15.8 P<0.000001). Candidate genes in this interval include Ptgs1, Dpp4, Ssb and Hc.

References

• Original: J:53356 McIndoe RA, et al., Localization of non-Mhc collagen-induced arthritis susceptibility loci in DBA/1j mice. Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):2210-4
• All: 1 reference(s)