Plcd4^tm1Kfu
Targeted Allele Detail

Summary

• Symbol: Plcd4^tm1Kfu
• Name: phospholipase C, delta 4; targeted mutation 1, Kiyoko Fukami
• MGI ID: MGI:2155167
• Synonyms: PLCdelta4-
• Gene: Plcd4 Location: Chr1:74581175-74605137 bp, + strand Genetic Position: Chr1, 38.54 cM, cytoband C3-C5
• Alliance: Plcd4^tm1Kfu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:69105
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced exons 2-5, which encode the translation start site and the PH domain. Western blot analyses of brain and male reproductive tissues did not detect protein in homozygous mutant mice. Immunohistochemical studies using a polyclonal antibody did not detect protein in testes of 10 week old homozygous mutant male mice. (J:69105)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Plcd4 Mutation: 39 strains or lines available

References

• Original: J:69105 Fukami K, et al., Requirement of phospholipase Cdelta4 for the zona pellucida-induced acrosome reaction. Science. 2001 May 4;292(5518):920-3
• All: 4 reference(s)