Ap6q^DBA/2J
QTL Variant Detail

Summary

• QTL variant: Ap6q^DBA/2J
• Name: alcohol preference 6 QTL; DBA/2J
• MGI ID: MGI:2155428
• QTL: Ap6q Location: Chr3:100372912-147045722 bp Genetic Position: Chr3, cM position of peak correlated region/allele: 43.71 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: DBA/2J

Variant description

• Allele Type: QTL
• Inheritance: Other (see notes)

Notes

Ap6q exhibits an additive mode of inheritance.

Ap2q and Ap6q participate in epistatic interactions. When Ap6q is homozygous for DBA/2J-derived alleles the genotype at Ap2q exerts little effect. However, homozygosity for C57BL/6J-derived alleles at both Ap2q and Ap6q results in a dramatic increase for alcohol preference.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:49707

Genome scan was performed on 218 (C57BL/6J x DBA/2J)F2 intercross animals using 101 microsatellite markers to identify QTLs associated with alcohol preference. Parental strain C57BL/6J exhibits higher alcohol preference compared to parental strain DBA/2J. Significant alcohol preference QTLs mapped to mouse Chromosome 1 spanning 45 cM - 95 cM (Ap1q, LOD = 4.5 between D1Mit194 and D1Mit110), mouse Chromosome 4 spanning 65 cM - telomere (Ap3q, LOD = 4.7 at D4Mit42), and mouse Chromosome 9 spanning 10 cM - 35 cM (Apq5, LOD = 4.8 cM at D9Mit330). Suggestive QTLs mapped to mouse Chromosome 2 spanning 30 cM - 50 cM (Ap2q, LOD = 3.1 at D2Mit61), mouse Chromosome 10 (Ap4q) spanning 18 cM - 57 cM, and mouse Chromosome 3 spanning 38 cM - 60 cM (Ap6q, LOD = 3.4 between D3Mit12 and D3Mit86). Ap1q, Ap2q, Ap3q, and Ap4q were provisionally mapped in BXD RI strains and confirmed in the present study, while Ap5q and Ap6q are novel. C57BL/6J-derived alleles increases alcohol preference at all loci mapped. Ap1q is follows adominant mode of inheritance and maps near Chrnd (Acrd), Chrng (Acrg), and Htr5b. Ap2q is consistent with a recessive or additive mode of inheritance and maps near Scn1a, Scn2a, Scn3a, and Scn7a . Ap3q is consistent with a dominant or additive mode of inheritance and maps near Htr1da, Htr1db, Ahd1 (Ssdh1), and Tas1r3 (Sac). Ap5q is consistent with a dominant or additive mode of inheritance and maps near Htr1b and Drd2. Ap6q is most consistent with an additive mode of inheritance and maps near the alcohol dehydrogenase gene complex at approximately 71.2 cM on mouse Chromosome 3.

J:71165

Several alcohol preference QTL (Ap1q, Ap2q, Ap3q, Ap4q, Ap5q, Ap6q) were mapped in a previous study by Tarantino et al, 1998 (J:49707). Parental strain C57BL/6J exhibits higher alcohol preference compared to parental strain DBA/2J. In this study, genotype data from the (C57BL/6J x DBA/2J)F2 intercross population was reanalyzed for epistatic interactions. Significant interaction was detected between markers mapping to the Ap2q interval on mouse Chromosome 2 and the Ap6q interval on mouse Chromosome 3. Markers D2Mit61 (34 cM) and D3Mit12 (49.2 cM) and markers D2Mit94 (48.1 cM) and D3Mit12 (49.2 cM) show the greatest evidence for epistatic interaction (P=0.0015 and P=0.0006, respectively). It appears Ap6q is epistatic to Ap2q. When Ap6q is homozygous for DBA/2J-derived alleles the genotype at Ap2q has little effect on the phenotype. But homozygosity for C57BL/6J-derived alleles at both Ap2q and Ap6q confers the greatest increase in alcohol preference.

References

• Original: J:49707 Tarantino LM, et al., Confirmation of quantitative trait loci for alcohol preference in mice. Alcohol Clin Exp Res. 1998 Aug;22(5):1099-105
• All: 2 reference(s)