Hsf1^tm1Ijb
Targeted Allele Detail

Summary

• Symbol: Hsf1^tm1Ijb
• Name: heat shock factor 1; targeted mutation 1, Ivor J Benjamin
• MGI ID: MGI:2155444
• Synonyms: Hsf1^-
• Gene: Hsf1 Location: Chr15:76361562-76385355 bp, + strand Genetic Position: Chr15, 35.95 cM
• Alliance: Hsf1^tm1Ijb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73593
• Parent Cell Line: KG-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 1.8kb genomic fragment containing all or part of 6 exons was replaced by a neomycin resistance cassette. These sequences encode half the DNA binding domain and the adjacent oligomerization domain of the protein. Northern blot analysis on RNA derived from homozygous embryonic cells demonstated that a mutant, truncated transcript was produced from this allele. However, western blot analysis indicated that no protein was detectable in cells derived from homozygous embryos. (J:73593)

Disease models

Expression

In Mice Carrying this Mutation:	78 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hsf1 Mutation: 25 strains or lines available

References

• Original: J:73593 McMillan DR, et al., Targeted disruption of heat shock transcription factor 1 abolishes thermotolerance and protection against heat-inducible apoptosis. J Biol Chem. 1998 Mar 27;273(13):7523-8
• All: 55 reference(s)