Mdfi^tm1Krt
Targeted Allele Detail

Summary

• Symbol: Mdfi^tm1Krt
• Name: MyoD family inhibitor; targeted mutation 1, Norbert Kraut
• MGI ID: MGI:2155446
• Synonyms: I-mfa null
• Gene: Mdfi Location: Chr17:48126253-48145616 bp, - strand Genetic Position: Chr17, 23.99 cM
• Alliance: Mdfi^tm1Krt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:50877
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced exon 5 coding sequences, corresponding to amino acids 162-246 required for protein-protein interactions with MyoD family members. Northern blot and RT-PCR analysis on RNA derived from E14.5 embryos demonstrated that no stable transcript was produced from this allele. (J:50877)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mdfi Mutation: 17 strains or lines available

References

• Original: J:50877 Kraut N, et al., Requirement of the mouse I-mfa gene for placental development and skeletal patterning. EMBO J. 1998 Nov 2;17(21):6276-88
• All: 3 reference(s)