Smad1^tm1Rob
Targeted Allele Detail

Summary

• Symbol: Smad1^tm1Rob
• Name: SMAD family member 1; targeted mutation 1, Elizabeth J Robertson
• MGI ID: MGI:2155483
• Synonyms: Smad1^Robm1
• Gene: Smad1 Location: Chr8:80065024-80126057 bp, - strand Genetic Position: Chr8, 37.56 cM
• Alliance: Smad1^tm1Rob page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71998
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neomycin resistance cassette replaced most of the first coding exon and 30bp of 3' flanking sequence, including the initiation codon and sequences encoding most of the MH1 domain. Northern analysis of 9.5dpc homozygous embryos detected a shorter transcript, but not the wild-type transcript, using a probe for the 3' UTR. Western blot analysis using an antibody that recognizes the MH2 domain did not detect intact or truncated protein in 9.5dpc homozygous embryos. (J:71998)

Expression

In Mice Carrying this Mutation:	28 assay results
In Structures Affected by this Mutation:	10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Smad1 Mutation: 32 strains or lines available

References

• Original: J:71998 Tremblay KD, et al., Mouse embryos lacking Smad1 signals display defects in extra-embryonic tissues and germ cell formation. Development. 2001 Sep;128(18):3609-21
• All: 3 reference(s)