Smad1^tm2.1Rob
Targeted Allele Detail

Summary

• Symbol: Smad1^tm2.1Rob
• Name: SMAD family member 1; targeted mutation 2.1, Elizabeth J Robertson
• MGI ID: MGI:2155485
• Synonyms: Smad1^Robcn
• Gene: Smad1 Location: Chr8:80065024-80126057 bp, - strand Genetic Position: Chr8, 37.56 cM
• Alliance: Smad1^tm2.1Rob page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71998
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated by crossing mice carrying the Smad1^tm2Rob allele to mice carrying a Cre transgene controlled by a mouse protamine 1 promoter that directs Cre-recombinase expression specifically to the male germline. An in vivo Cre-mediated excision removed all of the first coding exon and approximately 2kb of 5' and 3' flanking genomic sequences, and left a single loxP and EcoRI site in the locus. Northern analysis of 9.5dpc homozygous embryos using a probe for the 3' UTR detected a shorter transcript, but not the wild-type transcript. Western blot analysis using an antibody that recognizes the MH2 domain did not detect intact or truncated protein in 9.5dpc homozygous embryos. (J:71998)
• Inheritance: Recessive

Disease models

Expression

In Mice Carrying this Mutation:

35 assay results

Tumor Data

List all tumor models in MMHCdb carrying Smad1^tm2.1Rob

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Smad1 Mutation: 32 strains or lines available

References

• Original: J:71998 Tremblay KD, et al., Mouse embryos lacking Smad1 signals display defects in extra-embryonic tissues and germ cell formation. Development. 2001 Sep;128(18):3609-21
• All: 4 reference(s)