Chrm5^tm1Jwe
Targeted Allele Detail

Summary

• Symbol: Chrm5^tm1Jwe
• Name: cholinergic receptor, muscarinic 5; targeted mutation 1, Jurgen Wess
• MGI ID: MGI:2155599
• Synonyms: M5R -
• Gene: Chrm5 Location: Chr2:112309516-112311114 bp, - strand Genetic Position: Chr2, 57.02 cM
• Alliance: Chrm5^tm1Jwe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:72953
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A genomic fragment containing sequences encoding the first 250 amino acids of the protein was replaced with a neomycin resistance cassette. RT-PCR analysis demonstrated that the transcript was not detectable in RNA derived from tissues of homozygous mice. (J:72953)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Chrm5 Mutation: 40 strains or lines available

References

• Original: J:72953 Yamada M, et al., Cholinergic dilation of cerebral blood vessels is abolished in M(5) muscarinic acetylcholine receptor knockout mice. Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):14096-101
• All: 23 reference(s)