Chrna7^tm2Bay
Targeted Allele Detail

Summary

• Symbol: Chrna7^tm2Bay
• Name: cholinergic receptor, nicotinic, alpha polypeptide 7; targeted mutation 2, Baylor College of Medicine
• MGI ID: MGI:2155604
• Synonyms: L250T
• Gene: Chrna7 Location: Chr7:62748440-62862274 bp, - strand Genetic Position: Chr7, 34.47 cM
• Alliance: Chrna7^tm2Bay page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:61536
• Parent Cell Line: AB2.1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted
• Mutation: Nucleotide substitutions
• Mutation details: Point mutations were introduced into exon 8 that altered codon 250 from one encoding leucine to one encoding threonine. Additional mutations in intron 7 created a new restriction site. A loxP-flanked neomycin and thymidine kinase selection cassette was also inserted in intron 9, but was subsequently removed by Cre mediated recombination in ES cells prior to the production of chimeric mice. (J:61536)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Chrna7 Mutation: 50 strains or lines available

References

• Original: J:61536 Orr-Urtreger A, et al., Mice homozygous for the L250T mutation in the alpha7 nicotinic acetylcholine receptor show increased neuronal apoptosis and die within 1 day of birth. J Neurochem. 2000 May;74(5):2154-66
• All: 6 reference(s)