Pbx1^tm1Mlc
Targeted Allele Detail

Summary

• Symbol: Pbx1^tm1Mlc
• Name: pre B cell leukemia homeobox 1; targeted mutation 1, Michael L Cleary
• MGI ID: MGI:2155612
• Synonyms: Pbx1-
• Gene: Pbx1 Location: Chr1:167946933-168259839 bp, - strand Genetic Position: Chr1, 75.95 cM
• Alliance: Pbx1^tm1Mlc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71996
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Insertion of a PGK-neomycin resistance cassette into exon 3, upstream of sequences encoding the homeodomain, disrupted the gene. Western blot analysis using a monoclonal antibody specific for the most abundunt isoform of the protein did not detect any protein product in homozygous mutant E16 embryos. Immunohistochemical analysis using this same antibody did not detect nuclear or cytoplasmic expression in limb buds of E11.5 homozygous mutant embryos. (J:71996)

Disease models

Expression

In Mice Carrying this Mutation:	164 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	37 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pbx1 Mutation: 40 strains or lines available

References

• Original: J:71996 Selleri L, et al., Requirement for Pbx1 in skeletal patterning and programming chondrocyte proliferation and differentiation. Development. 2001 Sep;128(18):3543-57
• All: 30 reference(s)