Ada^tm1Vlr
Targeted Allele Detail

Summary

• Symbol: Ada^tm1Vlr
• Name: adenosine deaminase; targeted mutation 1, Dinko Valerio
• MGI ID: MGI:2155977
• Gene: Ada Location: Chr2:163568504-163592159 bp, - strand Genetic Position: Chr2, 84.44 cM, cytoband H3
• Alliance: Ada^tm1Vlr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:26731
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A hygromycin selection cassette replaced a genomic fragment containing exon 4, which encodes part of the active site of the enzyme. Western blot analysis of thymus, liver and brain extracts confirmed that the protein was not detectable in homozygous mice. Activity assays demonstrated that no functional protein was made from this allele in homozygous mice. (J:26731)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ada Mutation: 31 strains or lines available

References

• Original: J:26731 Migchielsen AA, et al., Adenosine-deaminase-deficient mice die perinatally and exhibit liver-cell degeneration, atelectasis and small intestinal cell death. Nat Genet. 1995 Jul;10(3):279-87
• All: 1 reference(s)