Plcg1^tm1Ine
Targeted Allele Detail

Summary

• Symbol: Plcg1^tm1Ine
• Name: phospholipase C, gamma 1; targeted mutation 1, Izumi Negishi
• MGI ID: MGI:2155982
• Synonyms: plc-gamma1-
• Gene: Plcg1 Location: Chr2:160573230-160617680 bp, + strand Genetic Position: Chr2, 80.97 cM
• Alliance: Plcg1^tm1Ine page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73254
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neomycin resistance cassette replaced approximately 17kb of sequence encoding the functional domains. (J:73254)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Plcg1 Mutation: 48 strains or lines available

Notes

Injection of mutant ES cells into wild-type (C57BL/6) blastocysts results in chimeric animals with distended abdomens, some of which display premature death at 3 months postpartum. Extensive chimerism (>70% agouti coat color) results in lethality. Chimeras with a 30-70% agouti coat color display: enlarged, polycystic kidneys, renal dysplasia (at 2 weeks), dilated renal tubules (at >2 weeks), and occasional glomerulosclerosis in high % chimeras with severe renal dysplasia. Mutant ES cells fail to contribute to lymphoid and hematopoietic organs (including thymus, spleen, bone marrow and red blood cells) in chimeric mice (J:73254).

References

• Original: J:73254 Shirane M, et al., Deficiency of phospholipase C-gamma1 impairs renal development and hematopoiesis. Development. 2001 Dec;128(24):5173-80
• All: 1 reference(s)