Adora3^tm1Jbsn
Targeted Allele Detail

Summary

• Symbol: Adora3^tm1Jbsn
• Name: adenosine A3 receptor; targeted mutation 1, Marlene A Jacobson
• MGI ID: MGI:2155986
• Synonyms: A3^-, A3AR-, A3KO, A3R^-
• Gene: Adora3 Location: Chr3:105811737-105816244 bp, + strand Genetic Position: Chr3, 46.45 cM, cytoband F3
• Alliance: Adora3^tm1Jbsn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:60293
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced a genomic fragment containing the initiation codon through sequences encoding the third transmembrane domain and the following intron. No transcript was detectable in northern blot analysis on RNA from bone marrow-derived mast cells (BMMCs) derived from homozygous mice. Binding assays confirmed the absence of protein from BMMCs derived from homozygous mice. (J:60293)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Adora3 Mutation: 24 strains or lines available

References

• Original: J:60293 Salvatore CA, et al., Disruption of the A(3) adenosine receptor gene in mice and its effect on stimulated inflammatory cells. J Biol Chem. 2000 Feb 11;275(6):4429-34
• All: 46 reference(s)