Bw13^DBA/2
QTL Variant Detail

Summary

• QTL variant: Bw13^DBA/2
• Name: body weight QTL 13; DBA/2
• MGI ID: MGI:2156044
• QTL: Bw13 Location: unknown Genetic Position: Chr5, Syntenic

Variant origin

• Strain of Specimen: DBA/2

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers decreased body weight compared to DU6i.
• Inheritance: Other (see notes)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Notes

Candidate Genes

J:91835

The correlation between gene expression and dietary interaction was examined in a population of (BALB/cStCrlfC3H/Nctr x VY/WffC3Hf/Nctr-A(vy)/a)F1 animals. A/a and A/A^vy animals were placed on a 70% (calorie restricted) or 100% (non-restricted) diet andliver mRNA levels were assessed for over 18,000 genes using DNA microarrays. Twenty-eight known genes showing statistically significant differential expression between the 70% and 100% calorie diets and A/a and A/A^vy genotypes mapped near known diabesity QTLs. These genes may be considered further for candidate genes.

On mouse Chromosome 5, Pdgfra (42 cM) showed differential expression between A/a and A/A^vy animals on the unrestricted diet. Diabesity QTLs Dbsty2 (45 cM), Bw8 (42 cM), and Bwob (44 cM) map near this candidate gene. Another candidate gene, Shrm (52 cM), showed differential expression between the restricted and unrestricted diets on the A/a background as well as between A/a and A/A^vy animals on the restricted diet. Diabesity QTL Igfbp3q1 (58 cM) maps near Shrm. Mdh2 (78 cM) shows differential expression between the restricted and unrestricted diets on a A/A^vy background. Diabesity QTL Bw13 (81 cM) maps near candidate gene Mhd2.

J:123128

A search for candidate genes was performed using DU6 and DU6i high body weight selection lines and lean unselected lines DUKs and DBA/2. RNA from epididymal fat tissue of 20 males from each line (DU6, DU6i, DUKs and DBA2/J) was pooled for analysis in Affymetrix GeneChips and resulted in the identification of 77 differentially expressed candidate genes. Of these, 14 genes are located in QTLs intervals affecting body weight and abdominal fat weight.

On mouse Chromosome 4, candidate gene Ak2 (61 cM) is upregulated in selected lines while candidate gene Ubxd5 at 65.7 cM (formerly 4930506L07Rik) is downregulated in selected lines. These genes map to the Afw14 QTL interval at 66 cM.

On mouse Chromosome 5, candidate gene Mcm7 is downregulated in selected lines compared to unselected lines. This gene is located near the Afw15 (80 cM) and Bw13 (81 cM) QTL intervals.

On mouse Chromosome 7, candidate genes Ldhc (23.5 cM) and Stard10 exhibit decreased expression in selected versus unselected lines. These genesare located near the Afw9 (23 cM) QTL interval.

On mouse Chromosome 11, candidate gene Prkca (68 cM) is upregulated in selected lines versus unselected lines. Candidate genes Ero1lb (formerly 1300013B24Rik-*note the current location of this gene according to assembly data inthe Mouse Genome Database is chromosome 13) and EST AA178476 map to the Bw4 QTL interval at 55 cM and display increased expression in selected lines compared to unselected lines. Zpbp is down-regulated in selected lines compared to unselected lines and maps to the Afw5 (10 cM) and Bw16 (14 cM) QTL intervals.

On mouse Chromosome 12, candidate gene Serpina3c maps near the Afw10 (21 cM) and Bw9 (17 cM) QTL intervals and is upregulated in selected lines. These genes are upregulated in selected lines compared to unselected lines. Candidate gene Cox8a (*note- the current location of this gene according to data in the Mouse Genome Database is chromosome 19) also maps to the same QTL intervals but is downregulated in selected lines comparedto unselected lines. Candidate gene Igh-1a (58 cM) maps near QTL Afw10 and is upregulated in selected lines compared to unselected lines.

On mouse Chromosome 13, candidate genes Hfe (15 cM) and Hist1h1c (formerly H1f2) map near the Afw18 QTL interval (11cM). These genes are upregulated in selected lines compared to unselected lines.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:66233

QTL effecting body weight, obesity and muscle weight were found on mouse Chromosomes 1, 4, 5, 7, 11, 12, 13, and 17 and for leptin on 14 and insulin on chromosome 4. The cross used in the genome wide scan included approximately 400 F2 mice from a (DU6i xDBA/2)F1 intercross. DU6i is a strain selected for high body weight. 93 microsatellite markers were analyzed in the genome wide scan.

Locations of the QTLs were provided by the authors and may not be equivalent to the closest microsatellites. Abdominalfat weight QTL 3, body weight QTL 13 and liver weight QTL 2 were mapped to Chromosome 5 to positions 80, 81 and 81 respectively.

10.03.2014 - Curator Note: We had originally associated the abdominal fat weight QTL 3 identified here, mapping to 80cM on mouse Chr 5, as Afw3. Afw3 was originally mapped using a different cross, an outbred cross of DU6 x DUK, in J:49578 in 1998. We consider the current mapping study a separate mapping experiment and have named the QTL identified here as Afw15.

10.06.2015 - Curator Note : Because Lwq2 (liver weight QTL 2) was originally mapped in J:49578 in 1998 using an outbred cross of DU6 x DUK, which differs from the cross used here, we consider the current study a separate mapping experiment and have named this QTL Lwq11.

References

• Original: J:66233 Brockmann GA, et al., Single QTL effects, epistasis, and pleiotropy account for two-thirds of the phenotypic F(2) variance of growth and obesity in DU6i x DBA/2 mice. Genome Res. 2000 Dec;10(12):1941-57
• All: 1 reference(s)