Adam3^tm1Ihgg
Targeted Allele Detail

Summary

• Symbol: Adam3^tm1Ihgg
• Name: ADAM metallopeptidase domain 3; targeted mutation 1, Institut fur Humangenetik
• MGI ID: MGI:2156166
• Synonyms: Cyrn -
• Gene: Adam3 Location: Chr8:25167241-25215868 bp, - strand Genetic Position: Chr8, 13.09 cM, cytoband A2
• Alliance: Adam3^tm1Ihgg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:58730
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A genomic fragment containing exon 3 was replaced with a neomycin selection cassette in opposite transcriptional orientation to the gene. RT-PCR analysis detected the presence of a trancript that spliced exon 2 to 4, but this mutation introduces a frameshift and premature stop codon. Western blots on testicular extracts confirmed the absence of the full length protein in homozygous mice. (J:58730)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Adam3 Mutation: 61 strains or lines available

References

• Original: J:58730 Shamsadin R, et al., Male mice deficient for germ-cell cyritestin are infertile. Biol Reprod. 1999 Dec;61(6):1445-51
• All: 3 reference(s)