Ext1^{Gt(LST064)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Ext1^{Gt(LST064)Byg}
• Name: exostosin glycosyltransferase 1; gene trap LST064, BayGenomics
• MGI ID: MGI:2156190
• Synonyms: Ext1^{Gt(pGT1.8TM)64Wcs}, Ext1^{Gt(pGT2TMpfs)064Wcs}
• Gene: Ext1 Location: Chr15:52931657-53209579 bp, - strand Genetic Position: Chr15, 20.0 cM
• Alliance: Ext1^{Gt(LST064)Byg} page

Mutation origin

• Mutant Cell Line: LST064
• Germline Transmission: Earliest citation of germline transmission: J:70273
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT2TMpfs
• Mutation details: The gene trap was inserted between exons 1 and 2, resulting in a fusion transcript of exon 1 fused to the gene trap vector sequence. The vector, pGT2TMpfs, contained part of En2, the transmembrane domain of CD4, Beta-geo, IRES, PLAP and pA elements. Northern blot against a cDNA probe for the 3' end of Ext1 showed no 3' mRNA present in mutants. This allele is reported to be a hypomorph (J:92212). (J:91757)
• Inheritance: Recessive
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  LST064	CC144574 ; (MGI Seq Detail)	5' RACE	Chr15:53207797-53207977 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  15 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ext1 Mutation: 65 strains or lines available

References

• Original: J:70273 Mitchell KJ, et al., Functional analysis of secreted and transmembrane proteins critical to mouse development. Nat Genet. 2001 Jul;28(3):241-9
• All: 8 reference(s)